Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and major immunoregulatory cytokine. It is a non-neutralizing antibody suitable for intracellular staining followed by flow cytometric analysis.

This antibody is pre-diluted and tested using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume).
Applicable cell number range: 10⁵–10⁸ cells/test.

Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.

For panels containing multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product #SB-4401-42), or
• Brilliant Stain Buffer™ (Product #00-4409-75)
  to minimize non-specific polymer interactions.

Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

Interleukin-2 (IL-2) is an immunomodulatory cytokine essential for:

• Proliferation of activated T cells
• Differentiation of B cells, NK cells, monocytes, and macrophages

IL-2 signals through the heterotrimeric IL-2 receptor (IL-2R), sharing its gamma chain with IL-4 and IL-7.
Monoallelic expression of IL-2 in mature thymocytes ensures precise gene regulation.
Disruption of IL-2-like genes in mice leads to ulcerative colitis-like disease, highlighting its critical role in immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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