Thermo Fisher Scientific PU.1 Recombinant Rabbit Monoclonal Antibody (BLR179J)
PU.1 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF, Flow, IP 등 다양한 응용에 적합. 인간 및 생쥐 시료에 반응하며, 고순도 정제 및 1 mg/mL 농도로 제공. 연구용으로 세포 발현 및 전사 조절 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL/10⁶ cells |
| Immunoprecipitation (IP) | 6 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR179J |
| Immunogen | Peptide representing a region between residue 220 and the C-terminus (residue 270) of Human transcription factor PU.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development.
The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes and regulates their expression in coordination with other transcription factors and cofactors.
The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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