Thermo Fisher Scientific CD31/PECAM-1 Recombinant Rabbit Monoclonal Antibody (BLR180J)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:100-1:500

-

Flow Cytometry (Flow)

0.25 µL/10^6 cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR180J

Immunogen

Recombinant protein representing the extracellular domain of mouse CD31 (aka, platelet endothelial cell adhesion molecule, residues 18-591). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD31 (PECAM-1),uniProtId:Q08481-1,ncbiNodeId:10090,antigenRange:18-591,antigenLength:727,antigenImageFileName:A700-180_CD31_PECAM-1_Q08481-1_Rabbit.svg,antigenImageFileNamePDP:A700-180_CD31_PECAM-1_Q08481-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

BBS, pH 8.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.