Thermo Fisher Scientific PU.1 Recombinant Rabbit Monoclonal Antibody (BLR178J)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Flow Cytometry (Flow)

1 µL/10^6 cells

-

Immunoprecipitation (IP)

12 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR178J

Immunogen

Peptide representing a region between residue 1 and 50 of Human transcription factor PU.1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PU.1,uniProtId:P17947-1,ncbiNodeId:9606,antigenRange:1-50,antigenLength:270,antigenImageFileName:A700-178_PU.1_P17947-1_Rabbit.svg,antigenImageFileNamePDP:A700-178_PU.1_P17947-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

500 µg/mL

Purification

purified

Storage buffer

BBS, pH 8.2, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunocytochemistry: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Target Information

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.