
Thermo Fisher Scientific HO-1 Monoclonal Antibody (1F12-A6), APC
HO-1 단백질을 검출하기 위한 APC 결합 단클론 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. 인간 및 여러 종에 반응하며, 높은 특이성과 안정성을 제공합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific HO-1 Monoclonal Antibody (1F12-A6), APC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F12-A6 |
| Immunogen | Human HO-1 synthetic peptide, amino acids 1–30 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931606 |
Additional Formats
- FITC (MA5-45153)
- PerCP (MA5-45154)
- Request custom conjugation
Product Specific Information
- 1 µg/mL was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
- Detects 32 kDa.
- Does not cross-react with HO-2.
Target Information
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules biliverdin and carbon monoxide. HO consists of two homologous isozymes: inducible HO-1 and constitutively expressed HO-2. HO-1 is induced by oxidative stress, inflammatory agents, transforming growth factor beta, and heat shock. Increased HO-1 expression acts as a cellular defense mechanism against oxidative stress by generating bilirubin, an antioxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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