Thermo Fisher Scientific CaMKII alpha/beta Monoclonal Antibody (22B1), PerCP
CaMKII α/β 인산화형 단백질을 인식하는 PerCP 결합 단일클론 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트 시료 반응성. 단백질 G 정제, 보존제 무첨가. 연구용 전용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100–1:5,000 | |
| Immunocytochemistry (ICC/IF) | 1:1,000 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 22B1 |
| Immunogen | Synthetic peptide |
| Conjugate | PerCP |
| Excitation/Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931602 |
Additional Formats
Product Specific Information
- 1 µg/mL was sufficient for detection of 0.2 µg CaMKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
- Detects phosphorylated CaMKII from rat tissues.
- Specific for alpha and beta subunits of CaMKII only when phosphorylated at Thr-286/287 (in beta).
Target Information
Ca²⁺/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium/calmodulin-dependent protein kinase that mediates cellular responses to various intercellular signals. It regulates synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca²⁺ homeostasis.
Phosphorylation of Thr286 activates CaM Kinase II autonomously, while phosphorylation at Thr305 inhibits its activity and reduces its association with the PSD, affecting long-term potentiation (LTP) and learning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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