Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 661
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands, and kidney. Homozygous knockout of Gata-3 is embryonic lethal, indicating its essential role.

Gata-3 is crucial for T cell commitment and survival. In the thymus, expression is mainly on CD4 single-positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter and represses T-bet expression, inhibiting Th1 differentiation.

Alternative splice variants have been reported, especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody recognizes both 50 and 45 kDa forms of the protein.

Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.

Target Information

The genes for all four subunits of the T-cell antigen receptor (alpha, beta, gamma, and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element via binding of GATA3 to enhancer elements of all four TCR genes. GATA3, containing a zinc finger domain, is expressed in T cells but not in B cells or macrophages, indicating its role in T-cell receptor gene activation.

Labastie et al. (1994) cloned the human gene and the 5′ end of the mouse gene. The human gene comprises six exons distributed over 17 kb of DNA, with two zinc fingers encoded by separate exons highly conserved with those of GATA1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.