Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), eBioscience
Gata-3 단백질을 인식하는 TWAJ 클론의 Rat IgG2b 단일클론 항체로, 인간 및 마우스 반응성을 가집니다. Western blot, ICC/IF, IHC 등 다양한 응용에 적합하며, Th2 분화 및 T세포 발달 연구에 유용합니다. 액상형, 0.5 mg/mL, 4°C 보관.
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 3 publications |
| Flow Cytometry (Flow) | - | View 47 publications |
| Miscellaneous PubMed (Misc) | - | View 3 publications |
| Mass Spectrometry (MS) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rhesus monkey |
| Published Species | Bat, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TWAJ |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865541 |
Available Conjugated Formats
- Alexa Fluor 488
- Alexa Fluor 700
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 661
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands, and kidney. Homozygous knockout of Gata-3 is embryonic lethal, indicating its essential role.
Gata-3 is crucial for T cell commitment and survival. In the thymus, expression is mainly on CD4 single-positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter and represses T-bet expression, inhibiting Th1 differentiation.
Alternative splice variants have been reported, especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody recognizes both 50 and 45 kDa forms of the protein.
Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.
Target Information
The genes for all four subunits of the T-cell antigen receptor (alpha, beta, gamma, and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element via binding of GATA3 to enhancer elements of all four TCR genes. GATA3, containing a zinc finger domain, is expressed in T cells but not in B cells or macrophages, indicating its role in T-cell receptor gene activation.
Labastie et al. (1994) cloned the human gene and the 5′ end of the mouse gene. The human gene comprises six exons distributed over 17 kb of DNA, with two zinc fingers encoded by separate exons highly conserved with those of GATA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.