Thermo Fisher Scientific CD273 (B7-DC) Monoclonal Antibody (122), eBioscience

Thermo Fisher Scientific CD273 (B7-DC) Monoclonal Antibody (Clone 122), eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.5 µg/test
  A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL (10⁵–10⁸ cells/test).

Product Specifications

Available Conjugated Formats

• FITC
• PE
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The 122 monoclonal antibody reacts with mouse B7-DC (PD-L2), a member of the B7 family within the Ig superfamily. B7-DC (~25 kDa) has a short cytoplasmic tail (4aa) and is primarily expressed by dendritic cells and monocytes/macrophages.
Unlike other B7 family members, B7-DC does not bind CD28/CTLA-4 but acts as a ligand for PD-1, regulating T cell proliferation and activation depending on cellular state.
Clone 122 blocks binding of TY25 (Product # 14-5986), another anti-mouse B7-DC mAb.
Recommended for flow cytometric analysis of mouse dendritic cells or B7-DC transfected cells.

Target Information

Programmed death-ligand 2 (PD-L2, B7-DC) is a ligand for PD-1 and part of the inhibitory immune checkpoint pathway.

• Expression: Primarily in dendritic cells; inducible in other immune and non-immune cells.
• Regulation: Upregulated by IL-4, TNF-α, and IFN-γ.
• Affinity: PD-L2 binds PD-1 with 2–6× higher affinity than PD-L1.
• Function: Inhibits T cell activation and cytokine secretion (e.g., IFN-γ, IL-10); can also stimulate proliferation via PD-1–independent pathways.
• Additional receptor: RGMb, involved in respiratory tolerance and BMP/neogenin signaling.
• Human PD-L2 structure: 201-aa extracellular domain, 21-aa transmembrane domain, 32-aa cytoplasmic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.