Thermo Fisher Scientific FDC Monoclonal Antibody (CNA.42), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/Test

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

CNA.42

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865545

Product Specific Information

Description: The monoclonal antibody CNA.42 recognizes a unique carbohydrate epitope found on follicular dendritic cells (FDCs) from a variety of species. FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis. Staining with this CNA.42 monoclonal antibody is mainly restricted to the plasma membrane but in limited cases may also be cytoplasmic (as seen in mast cells).

This monoclonal has been shown to be a useful reagent for both flow cytometry and IHC analysis.

Applications Reported: This CNA.42 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemistry on frozen and formalin-fixed paraffin embedded tissue sections.

Applications Tested: This CNA.42 antibody has been tested by flow cytometric of a human cell line and western blotting. This can be used at less than or equal to 0.5 µg per million cells in a 100 µL total staining volume or 2 µg/mL (for western blotting). The antibody has also been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsil using high pH antigen antigen retrieval. For IHC applications, the antibody can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.