Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (MIH4), eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (MIH4), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• APC
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 645
• Request custom conjugation available

Product Specific Information

The MIH4 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes.

Two ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), have been identified for PD-1. These ligands show overlapping functions and are constitutively expressed on some normal tissues, with upregulation on activated antigen-presenting cells. The MIH4 antibody recognizes a different epitope than clone J105.

Applications Reported:

• Flow cytometric analysis
• Immunohistology staining of frozen tissue sections

Applications Tested:
Tested by flow cytometric analysis of PHA-stimulated peripheral blood mononuclear cells. Recommended usage ≤ 1 µg/test (100 µL final volume). Optimal titration is advised for specific assays.

Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

T-cell activation is regulated by inhibitory receptors such as PD-1 (PDCD-1), which mediate signals from antigen-presenting cells (APCs). PD-1 binds two ligands, PD-L1 and PD-L2, and inhibits immune activation in the absence of danger signals (e.g., LPS). PD-1 deficiency leads to hyperactivated immune responses and autoimmune disease. Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.