Thermo Fisher Scientific Ephrin B1 Monoclonal Antibody (25H11), eBioscience
Mouse Ephrin B1 인식 단클론 항체(25H11)로, 신경 및 혈관 발달 연구에 적합. IHC(F), ICC/IF, IP 등 다양한 응용 가능. 고순도(>90%) 및 낮은 응집도(<10%)로 안정적 성능 제공. PBS buffer에 보관, 4°C 저장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25H11 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219677 |
Product Specific Information
The monoclonal antibody 25H11 recognizes mouse Ephrin B1, a 47 kDa membrane protein. Ephrin B1 belongs to a family of ephrins composed of type A (linked to the cell membrane through a glycosylphosphatidyl inositol group) or type B (transmembrane) proteins.
Type B ephrins (B1, B2, B3) contain cytoplasmic domains with tyrosine residues that can be phosphorylated. Ephrin B and Eph B receptors interact to potentiate bidirectional downstream signaling between cells expressing either or both ligand and receptor.
Ephrins play roles in:
- Neural crest cell development
- Neurogenesis and neural migration
- Kidney and heart cyto-architecture development
Overexpression of type B ephrins in cancer cells is associated with increased vascularity and invasiveness.
Applications Reported:
- Immunohistochemical staining of frozen tissue sections
- Immunoprecipitation
Applications Tested:
Tested on frozen mouse kidney tissue sections at ≤10 µg/mL. Antibody should be titrated for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ephrin B1 is a cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases crucial for migration, repulsion, and adhesion during neuronal, vascular, and epithelial development.
It binds promiscuously to Eph receptors on adjacent cells, enabling contact-dependent bidirectional signaling.
Forward signaling occurs downstream of the receptor, while reverse signaling occurs downstream of the ephrin ligand.
Binding Partners: EPHA4, EPHA3, EPHB4
Biological Roles:
- Heart morphogenesis and angiogenesis (with EPHB4)
- Regulation of cell adhesion and migration
- Axon orientation control
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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