Thermo Fisher Scientific Ephrin B1 Monoclonal Antibody (25H11), eBioscience

Applications

Product Specifications

Product Specific Information

The monoclonal antibody 25H11 recognizes mouse Ephrin B1, a 47 kDa membrane protein. Ephrin B1 belongs to a family of ephrins composed of type A (linked to the cell membrane through a glycosylphosphatidyl inositol group) or type B (transmembrane) proteins.
Type B ephrins (B1, B2, B3) contain cytoplasmic domains with tyrosine residues that can be phosphorylated. Ephrin B and Eph B receptors interact to potentiate bidirectional downstream signaling between cells expressing either or both ligand and receptor.

Ephrins play roles in:

• Neural crest cell development
• Neurogenesis and neural migration
• Kidney and heart cyto-architecture development

Overexpression of type B ephrins in cancer cells is associated with increased vascularity and invasiveness.

Applications Reported:

• Immunohistochemical staining of frozen tissue sections
• Immunoprecipitation

Applications Tested:
Tested on frozen mouse kidney tissue sections at ≤10 µg/mL. Antibody should be titrated for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ephrin B1 is a cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases crucial for migration, repulsion, and adhesion during neuronal, vascular, and epithelial development.
It binds promiscuously to Eph receptors on adjacent cells, enabling contact-dependent bidirectional signaling.
Forward signaling occurs downstream of the receptor, while reverse signaling occurs downstream of the ephrin ligand.

Binding Partners: EPHA4, EPHA3, EPHB4
Biological Roles:

• Heart morphogenesis and angiogenesis (with EPHB4)
• Regulation of cell adhesion and migration
• Axon orientation control

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.