Thermo Fisher Scientific cGAS Monoclonal Antibody (HGAYN), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HGAYN

Immunogen

E. coli-derived recombinant human cGAS protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762638

Product Specific Information

Description: This HGAYN monoclonal antibody recognizes human cGAS. This antibody works on reduced lysates.

Applications Reported: This HGAYN antibody has been reported for use in Western Blot analysis.

Applications Tested: This HGAYN antibody has been tested by western blot of HeLa cell line. This may be used at less than or equal to 2 µg/mL.

Target Information

cGAS (Cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of 2, 5 phosphodiester linkage at the GpA step and the formation of 3, 5 phosphodiester linkage at the ApG step. This produces c[G(2,5)pA(35)p] responses. cGAS also binds cytosolic DNA directly, leading to the activation and synthesis of cGAMP. Then, a second messenger binds to and activates TMEM173/STING thereby triggering type-I interferon production. cGAS is also involved in innate immune sensorship of infection, detection of DNA from bacteria, response to cellular stress, DNA damage, and regulation of cellular senescence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.