Thermo Fisher Scientific CD8b Monoclonal Antibody (SIDI8BEE), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SIDI8BEE

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Brilliant UV 661
• Brilliant Violet 605
• eFluor 660
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865105

Product Specific Information

Description: The SIDI8BEE monoclonal antibody reacts with human and rhesus macaque CD8 beta. The CD8b chain associates with the CD8a chain to form the CD8ab heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. The CD8 receptor complex binds to MHC class I and plays a role in T cell development and activation of mature T cells. Long been thought to exist either as a heterodimer of the alpha and beta chain or as a homodimer of alpha chains, beta chains have now been shown to homodimerize as well. SIDI8BEE can detect the CD8 beta chain in both the CD8ab heterodimer and the CD8bb homodimer.

SIDI8BEE is cross-reactive to human and rhesus macaque CD8b. Other non-human primate species have not been tested.

Applications Reported: This SIDI8BEE antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and western blotting.

Applications Tested: This SIDI8BEE antibody has been tested by flow cytometric analysis of human peripheral blood lymphocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.