Thermo Fisher Scientific CD8b Monoclonal Antibody (SIDI8BEE), eBioscience
인간 및 원숭이 CD8β 인식 단일클론 항체로, 세포독성 T세포 분석에 적합합니다. Flow cytometry, Western blot, Immunoprecipitation 등에 사용 가능합니다. 고순도(>90%) 항체로 0.5 mg/mL 농도의 액상 형태로 제공됩니다.
- 카탈로그번호
- 14-5273-xx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD8b Monoclonal Antibody (SIDI8BEE), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | 5 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SIDI8BEE |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865105 |
Available Conjugated Formats
- Brilliant UV 661
- Brilliant Violet 605
- eFluor 660
- NovaFluor Blue 510
- NovaFluor Blue 585
- NovaFluor Blue 610-70S
- NovaFluor Blue 690
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 702
- Request custom conjugation available
Product Specific Information
The SIDI8BEE monoclonal antibody reacts with human and rhesus macaque CD8 beta.
The CD8β chain associates with CD8α to form the CD8αβ heterodimer expressed on thymocytes and peripheral cytotoxic T cells. The CD8 receptor complex binds to MHC class I molecules and plays a key role in T cell development and activation.
SIDI8BEE can detect CD8β in both CD8αβ heterodimers and CD8ββ homodimers. Cross-reactivity has been confirmed for human and rhesus macaque CD8β; other primates have not been tested.
Applications Reported: Flow cytometry, immunoprecipitation, and Western blotting.
Applications Tested: Flow cytometric analysis of human peripheral blood lymphocytes at ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration is recommended.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes, mediating cell-cell interactions within the immune system. Acting as a coreceptor with the T-cell receptor, CD8 recognizes antigens presented by MHC class I molecules on antigen-presenting cells.
The functional coreceptor can exist as a homodimer (αα) or heterodimer (αβ). Both chains share homology with immunoglobulin variable light chains. The CD8B gene encodes the β chain isoforms, with multiple alternatively spliced variants and a pseudogene located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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