Thermo Fisher Scientific Progesterone Receptor Monoclonal Antibody (KMC912), eFluor 660, eBioscience

Thermo Fisher Scientific Progesterone Receptor Monoclonal Antibody (KMC912), eFluor 660, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• Request custom conjugation

Product Specific Information

This KMC912 monoclonal antibody reacts with human progesterone receptor (PgR, PR), a nuclear receptor that regulates transcription in a ligand-dependent manner. The human PgR exists in alpha (94 kDa) and beta (120 kDa) forms, typically forming heterodimers. Binding of progestin induces conformational changes, enabling DNA binding through progestin response elements (PRE) and regulating gene expression.

PgR expression in breast tissue correlates with improved survival and endocrine therapy response. Altered PgRa:PgRb ratios are associated with cancer progression, including ductal carcinoma and uterine cancer.

Applications Reported: Suitable for intracellular staining, flow cytometry, immunohistochemistry (FFPE), microscopy, and immunocytochemistry.

Applications Tested:

• IHC (FFPE): ≤ 20 µg/mL (low pH antigen retrieval)
• ICC: ≤ 20 µg/mL (methanol-fixed cells)
• Flow cytometry: ≤ 1 µg/test (BT474 cells using Foxp3/Transcription Factor Staining Buffer Set, Product #00-5523-00)

Refer to BestProtocols®: Protocol B for intracellular nuclear proteins.

Note: Carefully titrate antibody for optimal performance.

Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647. Emits at 659 nm, excited by red laser (633 nm).

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The progesterone receptor (PR) is a steroid nuclear receptor mediating progesterone effects in reproduction. Exists as 94 kDa (Form A) and 120 kDa (Form B) proteins. PR-B activates genes for endometrial maintenance and ovulation inhibition, while PR-A acts as a transcriptional repressor due to an N-terminal deletion exposing an inhibitory domain.

Binding of progesterone induces receptor dimerization and DNA interaction at PRE sequences, activating transcription. Mutations in the PGR gene can cause reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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