Thermo Fisher Scientific Progesterone Receptor Monoclonal Antibody (KMC912), eFluor 660, eBioscience
인간 프로게스테론 수용체(PR)를 검출하는 KMC912 단클론 항체로, eFluor 660 형광체가 결합된 제품입니다. IHC, ICC/IF, Flow Cytometry에 적합하며, 고감도·특이적 결합 특성을 지닙니다. 4°C 암소 보관, 연구용으로만 사용됩니다.
- 카탈로그번호
- 50-9764-80
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Progesterone Receptor Monoclonal Antibody (KMC912), eFluor 660, eBioscience
Thermo Fisher Scientific Progesterone Receptor Monoclonal Antibody (KMC912), eFluor 660, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
| Flow Cytometry (Flow) | 1 µg/test |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KMC912 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574363 |
Additional Formats
Product Specific Information
This KMC912 monoclonal antibody reacts with human progesterone receptor (PgR, PR), a nuclear receptor that regulates transcription in a ligand-dependent manner. The human PgR exists in alpha (94 kDa) and beta (120 kDa) forms, typically forming heterodimers. Binding of progestin induces conformational changes, enabling DNA binding through progestin response elements (PRE) and regulating gene expression.
PgR expression in breast tissue correlates with improved survival and endocrine therapy response. Altered PgRa:PgRb ratios are associated with cancer progression, including ductal carcinoma and uterine cancer.
Applications Reported: Suitable for intracellular staining, flow cytometry, immunohistochemistry (FFPE), microscopy, and immunocytochemistry.
Applications Tested:
- IHC (FFPE): ≤ 20 µg/mL (low pH antigen retrieval)
- ICC: ≤ 20 µg/mL (methanol-fixed cells)
- Flow cytometry: ≤ 1 µg/test (BT474 cells using Foxp3/Transcription Factor Staining Buffer Set, Product #00-5523-00)
Refer to BestProtocols®: Protocol B for intracellular nuclear proteins.
Note: Carefully titrate antibody for optimal performance.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647. Emits at 659 nm, excited by red laser (633 nm).
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
The progesterone receptor (PR) is a steroid nuclear receptor mediating progesterone effects in reproduction. Exists as 94 kDa (Form A) and 120 kDa (Form B) proteins. PR-B activates genes for endometrial maintenance and ovulation inhibition, while PR-A acts as a transcriptional repressor due to an N-terminal deletion exposing an inhibitory domain.
Binding of progesterone induces receptor dimerization and DNA interaction at PRE sequences, activating transcription. Mutations in the PGR gene can cause reproductive abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.