Thermo Fisher Scientific LAMP5 (BAD-LAMP) Monoclonal Antibody (34.2), eFluor 660, eBioscience
인간 LAMP5(BAD-LAMP)를 인식하는 Rat IgG1 단클론 항체로 eFluor 660 형광 표지. ICC/IF, IHC, Flow Cytometry에 적합하며, 651/668 nm의 Excitation/Emission 특성. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
| Flow Cytometry (Flow) | 0.06 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 34.2 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651/668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% Sodium Azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574375 |
Additional Formats
Product Specific Information
The monoclonal antibody 34.2 recognizes human lysosome-associated membrane protein 5 (LAMP5), also known as brain and dendritic cell-associated LAMP-like molecule (BAD-LAMP).
In humans, BAD-LAMP is expressed in post-natal neurons and non-activated plasmacytoid dendritic cells. Expression in neurons is localized to intracellular vesicles distributed in specific microdomains along neurites and may play a role in endocytosis. BAD-LAMP is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of plasmacytoid dendritic cells and is lost upon TLR activation.
This antibody has been tested by:
- Intracellular staining and flow cytometric analysis of normal human peripheral blood cells (0.06 µg/test)
- Immunohistochemistry of formalin-fixed paraffin-embedded human tissue using low or high pH antigen retrieval (≤20 µg/mL)
eFluor® 660 is a replacement for Alexa Fluor® 647, excited with a red laser (633 nm) and emits at 659 nm. Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Lysosome-associated membrane protein 5 (LAMP5) is a member of the LAMP family, also known as BAD-LAMP. Its expression is restricted to post-natal neurons and non-activated plasmacytoid dendritic cells. In neurons, it is localized to vesicular microdomains and may function in endocytosis. In dendritic cells, BAD-LAMP resides in the ER-Golgi intermediate compartment and is lost upon TLR activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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