Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor 660, eBioscience
인간, 마우스, 랫트의 α-평활근 액틴을 인식하는 1A4 단클론 항체로, eFluor 660 형광체가 결합되어 있습니다. 면역조직화학, 면역세포화학, 유세포분석 등에 적합하며, 633 nm 레이저로 여기되어 668 nm에서 방출됩니다. Affinity chromatography로 정제된 액상 형태로 제공됩니다.
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Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor 660, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 2 publications |
| Immunohistochemistry (IHC) | 1:100 | 17 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–5 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 8 publications |
| Flow Cytometry (Flow) | - | 8 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A4 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574361 |
Product Specific Information
The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin (α-SMA), a 42 kDa protein that is a major component of the cytoskeletal structural network. This antibody is specific for the alpha form of muscle actin, expressed by smooth muscle cells, myofibroblasts, and myoepithelial cells. It is useful in identifying leiomyomas, leiomyosarcomas, pleomorphic adenomas, and as a prognostic marker for basal cell carcinoma.
Applications Reported:
Used in immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested:
- IHC (P): ≤5 µg/mL (low pH antigen retrieval)
- ICC/IF: ≤5 µg/mL (methanol-fixed and permeabilized cells)
Careful titration is recommended for optimal assay performance.
Fluorophore Information:
eFluor™ 660 replaces Alexa Fluor™ 647. Emits at 659 nm, excited by red laser (633 nm).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtered post-manufacturing with 0.2 µm filter.
Target Information
Smooth Muscle Actin (α-SMA) is part of the actin protein family, essential for cell motility, structure, and integrity. Alpha actin is a major constituent of the contractile apparatus, while beta and gamma isoforms regulate motility. α-SMA is found in smooth muscle vessel walls, gut wall, myometrium, and myoepithelial cells of breast and salivary glands. Defects in the α-SMA gene are associated with familial thoracic aortic aneurysm type 6. During wound healing and fibrocontractive diseases, α-SMA appears in stress fibers of fibroblasts.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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