Thermo Fisher Scientific KDEL Monoclonal Antibody (2D6)
KDEL 서열을 인식하는 Mouse monoclonal antibody로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot과 면역세포염색에 적합하며, 고순도의 Protein G 정제 항체입니다. PBS/glycerol buffer에 보관하며 -20°C에서 안정적으로 저장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D6 |
| Immunogen | KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735251 |
Available Conjugate Formats
Product Specific Information
A 1:1,000 dilution of MA5-27581 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects KDEL-tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL), or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
78 and 94 kDa glucose-regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence.
The presence of carboxy-terminal KDEL is necessary for ER retention and sufficient to reduce secretion of proteins from the ER. This retention is mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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