Thermo Fisher Scientific Metallothionein Monoclonal Antibody (2B5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B5

Immunogen

Full length Pseudomonas aeruginosa Metallothionein protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735268

Product Specific Information

A 1:1,000 dilution of MA5-27586 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.

Target Information

This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. The conserved cysteine residues co-ordinate metal ions using mercaptide linkages. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. Disruption of two metallothionein genes in mouse resulted in defects in protection against heavy metals, oxidative stress, immune reactions, carcinogens, and displayed obesity. MT1A (Metallothionein 1A) is a Protein Coding gene. Diseases associated with MT1A include Menkes Disease and Deficiency Anemia. Among its related pathways are Metal ion SLC transporters and Cellular responses to stimuli. An important paralog of this gene is MT2A.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.