Thermo Fisher Scientific Metallothionein Monoclonal Antibody (8D8)
Pseudomonas aeruginosa 유래 Metallothionein 단백질을 인식하는 Mouse monoclonal antibody. Western blot 및 ELISA에 사용 가능하며, 1:1,000 희석에서 검출 효율 우수. Protein G 정제, PBS/glycerol buffer로 안정화, 연구용 전용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution | Note |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D8 |
| Immunogen | Full length Pseudomonas aeruginosa Metallothionein protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735269 |
Product Specific Information
A 1:1,000 dilution of MA5-27587 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects a 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.
Target Information
Metallothionein family proteins are low molecular weight, cysteine-rich, and bind divalent heavy metal ions via mercaptide linkages. They function as antioxidants, protect against hydroxyl radicals, and maintain metal ion homeostasis. These proteins are also involved in detoxification of heavy metals. Disruption of metallothionein genes in mice leads to defects in protection against heavy metals, oxidative stress, immune responses, and carcinogens, and may cause obesity.
MT1A (Metallothionein 1A) is a protein-coding gene. Diseases associated with MT1A include Menkes Disease and Deficiency Anemia. Related pathways include metal ion SLC transporters and cellular responses to stimuli. MT2A is an important paralog.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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