Thermo Fisher Scientific KDEL Monoclonal Antibody (2C1)
KDEL 모노클로날 항체(2C1)는 인간, 마우스, 랫트 시료에서 KDEL 서열을 인식하며 ER 단백질 검출에 적합합니다. Western blot에 1:1,000 희석으로 사용 가능하며, 고순도 Protein G 정제 및 안정적인 PBS/glycerol 버퍼로 제공됩니다.
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Applications
- Western Blot (WB): Tested dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2C1 |
| Immunogen | KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735250 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-27580 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects KDEL tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence.
The presence of carboxy-terminal KDEL appears to be necessary for ER retention and sufficient to reduce secretion of proteins from the ER.
This retention is mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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