Thermo Fisher Scientific Phospho-B-Raf (Thr400) Polyclonal Antibody
B-Raf 단백질의 Thr400 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot 및 Dot blot에 사용 가능. 인간 시료에 반응하며, 서열 상 유사성으로 마우스에도 반응 예상. Protein A 및 항원 친화 크로마토그래피로 정제됨. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Dot Blot (DB) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T400 of human BRAF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980217 |
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and plays a role in postsynaptic responses of hippocampal neurons. This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion. Mutations in B-Raf are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of the lung. A pseudogene for B-Raf is located on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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