Thermo Fisher Scientific Phospho-LC3B (Thr12) Polyclonal Antibody
Rabbit polyclonal antibody specific for phosphorylated LC3B (Thr12). Validated for dot blot (1:500). Recognizes human LC3B and predicted to react with bovine. Suitable for autophagy research. Supplied as liquid, 0.38 mg/mL, stored at 4°C or -20°C.
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Applications and Tested Dilution
- Application: Dot blot (DB)
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T12 of human LC3B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.38 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2137744 |
Product Specific Information
This antibody is predicted to react with bovine based on sequence homology.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans is encoded by the MAP1LC3B gene. LC3B is associated with microtubule assembly and plays an important role in neurogenesis and autophagy.
Autophagy involves the degradation of cytoplasmic components via autophagosomes that fuse with lysosomes. LC3B is a key marker of this process and mediates interactions between microtubules and cytoskeletal elements.
LC3B is implicated in various biological processes and diseases, including cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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