Thermo Fisher Scientific Phospho-c-Raf (Ser339) Polyclonal Antibody
Ser339 인산화된 c-Raf 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot, ICC/IF, Dot blot에 사용 가능. Human 반응성, 다른 종에도 예측 반응. Protein A 및 항원 친화 크로마토그래피로 정제됨. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Dot blot (DB) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S339 of human RAF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980854 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, mouse, and rat based on sequence homology.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane-associated GTPases to which it binds directly. Once activated, Raf-1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate and activate the serine/threonine-specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in controlling gene expression involved in the cell division cycle, apoptosis, cell differentiation, and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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