Thermo Fisher Scientific Phospho-Huntingtin (Ser419) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Huntingtin only when phosphorylated at Ser419, which site has been historically referenced as Ser421.

Target Information

Huntingtin is a disease gene linked to Huntington’s disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. The disorder is caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, translating into a polyglutamine repeat in the protein product. HD typically manifests in mid-life and is an autosomal dominant disease characterized by psychiatric symptoms, dementia, and involuntary movements (chorea), leading to death within 10–20 years.

The huntingtin locus spans 180 kb and contains 67 exons. The gene is widely expressed and essential for normal development, producing two alternatively polyadenylated transcripts (~13.7 kb and ~10.3 kb) with differing tissue abundance. The larger transcript is predominantly found in brain tissues, while the smaller transcript is more widely expressed. The genetic defect may alter mRNA or protein function rather than abolish transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.