Thermo Fisher Scientific Phospho-HSF1 (Ser303) Polyclonal Antibody

Thermo Fisher Scientific Phospho-HSF1 (Ser303) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Antibody detects endogenous levels of HSF1 only when phosphorylated at Serine 303.

Target Information

All organisms respond to elevated temperatures and environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes.

The HSEs are highly conserved among species, yet HSF purified from yeast, Drosophila, and human have different molecular weights and do not show significant immunological cross-reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli:

• HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents.
• HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.

HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. During stress, HSF1 becomes activated to a nuclear localized, trimeric state that binds DNA. Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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