Thermo Fisher Scientific PGR Monoclonal Antibody (OTI20A11), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7 K MWCO, 0.5 mL, Product #89882).

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors and mediates the physiological effects of progesterone, essential for reproductive processes such as pregnancy establishment and maintenance.
PR exists as two isoforms: PR-A (94 kDa) and PR-B (120 kDa), generated by alternative translation initiation sites. PR-B is transcriptionally active, regulating genes involved in endometrium maintenance and inhibition of ovulation.
PR-A lacks 165 amino acids at the N-terminus, exposing an inhibitory domain that represses steroid hormone transcriptional activity.
In its inactive state, PgR forms a complex with heat shock proteins and immunophilins. Progesterone binding induces a conformational change, receptor dimerization, and binding to progesterone response elements (PRE), activating transcription.
A null mutation in the PGR gene can cause pleiotropic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.