Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Super Bright 780, eBioscience
마우스 CD4 분자를 인식하는 GK1.5 클론 단일클론 항체로, Super Bright 780 형광체 결합. Flow cytometry에 최적화되어 있으며, 405 nm excitation/780 nm emission 특성. 면역세포 분석 및 T세포 기능 연구용으로 적합.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Super Bright 780, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 92 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | Super Bright™ 780 |
| Excitation / Emission Max | 413 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2722965 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV/Violet/Yellow series
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue/Red/UV/Violet/Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702
- Request custom conjugation
Product Specific Information
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays an essential role in T-cell development and function.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes; ≤0.25 µg per test (100 µL final volume)
- Excitation/Emission: 405 nm / 780 nm (Violet laser)
- Recommended Filter: 780/60 bandpass
Notes:
- Protect from light; Super Bright 780 is sensitive to photo-induced oxidation.
- When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (SB-4401) to minimize non-specific interactions.
- Compensation may vary depending on bead type; set compensation using cells when possible.
- Samples can be fixed in IC Fixation Buffer (00-8222) or Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in dark with minimal brightness loss.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD4 antigen is a co-receptor for MHC class II molecules and HIV. It is primarily expressed in T-helper cells, monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. It plays a key role in T-cell activation and may mediate neuronal damage in immune-related CNS diseases.
(RefSeq, July 2017)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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