Thermo Fisher Scientific CD5 Monoclonal Antibody (UCHT2), Super Bright 780, eBioscience

Thermo Fisher Scientific CD5 Monoclonal Antibody (UCHT2), Super Bright 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by most thymocytes, mature T cells, and a subset of B cells. CD5 signaling activates T cells and mediates T-B cell interactions via CD72 binding.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on human peripheral blood cells; recommended 5 µL (0.5 µg)/test in 100 µL final volume
• Excitation / Emission: 405 nm / 780 nm (Violet Laser)
• Recommended Filter: 780/60 bandpass
• Light Sensitivity: Protect from light
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

For compensation setup, use stained cells rather than beads when using UltraComp eBeads (Product # 01-2222-42) or AbC Total Antibody Compensation Beads (Product # A10497).

Super Bright 780 is a tandem dye excited by the violet laser (405 nm) and emits at 780 nm. This dye is sensitive to photo-induced oxidation.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD5 is a 67 kDa human T-lymphocyte transmembrane glycoprotein expressed on most thymocytes, mature T cells, and some B cells. It functions as a receptor regulating T-cell proliferation and is associated with thymus cancer and Richter’s Syndrome.

Usage Note

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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