Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Super Bright 780, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Super Bright 780, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 585, 690
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702
• Custom conjugation available

Product Specific Information

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by thymocytes, T-helper cells, and monocytes. CD4 serves as a receptor for HIV. The OKT4 and RPA-T4 antibodies recognize different epitopes and do not cross-block each other.

This RPA-T4 antibody is pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended usage is 5 µL (0.125 µg) per test, defined as the amount required to stain a 100 µL sample containing 10⁵–10⁸ cells.

Super Bright 780 is a tandem dye excited by the violet laser line (405 nm) and emits at 780 nm. Use a 780/60 bandpass filter for optimal detection.

When using multiple Super Bright dye-conjugated antibodies, employ Super Bright Complete Staining Buffer (Product # SB-4401-42) to minimize non-specific polymer interactions.

Compensation Notes:
Higher compensation values may occur in the violet 450/50 channel when using UltraComp eBeads (Product # 01-2222-42) compared to single-color stained cells. In such cases, set compensation using cells. Similar observations may occur with AbC Total Antibody Compensation beads (Product # A10497).

Light Sensitivity:
Super Bright 780 is photo-sensitive; protect from light during storage and use.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation/Emission:
405 nm / 780 nm (Violet Laser)

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. It is mainly expressed in T-helper lymphocytes but also found in monocytes, macrophages, and dendritic cells. Tissue distribution includes thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. CD4 contributes to early T-cell activation and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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