Thermo Fisher Scientific IRF1 Recombinant Rabbit Monoclonal Antibody (SR44-08)
IRF1 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션. 인간, 마우스, 랫트 반응성.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:50 | - |
| Flow Cytometry (Flow) | 1:50 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR44-08 |
| Immunogen | Recombinant protein within Human IRF1 (aa 86–325) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809290 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
IRF1 (Interferon Regulatory Factor 1) encodes a transcription factor that activates interferons alpha and beta. In mouse models, IRF1 is required for double-stranded RNA induction of these genes.
IRF1 also activates genes induced by interferons alpha, beta, and gamma, and plays roles in apoptosis regulation and tumor suppression.
Diseases associated with IRF1 include gastric cancer (somatic) and lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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