Thermo Fisher Scientific RAB9 Recombinant Rabbit Monoclonal Antibody (SR45-05)
RAB9 단백질을 표적하는 Thermo Fisher의 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF 등에 적합합니다. 높은 특이성과 민감도를 제공하며, 로트 간 일관성과 동물 유래 성분이 없는 포뮬레이션으로 연구용에 최적화되었습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR45-05 |
| Immunogen | Synthetic peptide within Human Rab9 (aa 31–80) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809291 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Rab proteins are Ras-like GTPases involved in intracellular protein transport. Members of the rab family mediate docking and fusion of transport vesicles between cellular compartments.
Rab9 is essential for trafficking mannose 6-phosphate receptor between the late endosome and trans-Golgi network (TGN), facilitating receptor recycling and efficient cellular transport.
Rab9 requires prenylation by two geranylgeranyl groups at the C-terminus, enabling membrane binding. Approximately 10–20% of cellular Rab9 is GDP-bound and associated with GDI-alpha in the cytosol, which recycles Rab9 to its membrane of origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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