Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation available

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer. Activation of T and B cells rapidly upregulates CD69, indicating its role in lymphocyte development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes, ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
  Optimal titration recommended.

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

Staining Panels: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.

Fixation: Samples can be fixed using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates. Powered by Sirigen™.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions) containing N-linked oligosaccharides. It is expressed on activated platelets and various lymphocytes, involved in signal transduction and proliferation of T cells, NK cells, and platelets.
Associated diseases include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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