Thermo Fisher Scientific CD105 (Endoglin) Monoclonal Antibody (SN6), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD105 (Endoglin) Monoclonal Antibody (SN6), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The SN6 monoclonal antibody reacts with human CD105 (Endoglin), a ~90 kDa disulfide-linked homodimer expressed on vascular endothelial cells, bone marrow cells, and activated macrophages. CD105 is involved in adhesion and embryonic angiogenesis.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated on U937 cells
  • Use at 5 µL (0.25 µg) per test (100 µL final volume)
  • Cell count: 10⁵–10⁸ cells/test (empirical determination)

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is compatible with cytometers equipped with a 355 nm ultraviolet laser. Ensure instrument compatibility before use.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation:
Samples may be stored up to 3 days in the dark at 4°C using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD105 (Endoglin) is a Type I transmembrane protein highly expressed on human vascular endothelial cells. It forms an O- and N-glycosylated homodimer and binds TGF-β1 and TGF-β3 with high affinity. Upregulation of CD105 is observed in tumor vasculature and proliferating cells, marking it as a proliferation-associated endothelial marker.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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