Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 480
• Brilliant Violet 786
• eFluor 450
• FITC
• NovaFluor Blue 510
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor.
This 60–70 kDa transmembrane protein binds IL-3 with low affinity by itself and with high affinity when associated with CD131 (common beta chain).
CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells
  • Use at 5 µL (0.125 µg) per test (100 µL final volume, cell number 10⁵–10⁸/test)

Brilliant Ultra Violet™ 661 (BUV661) is a tandem dye that emits at 660 nm and is designed for use with cytometers equipped with a 355 nm UV laser.
Ensure your instrument supports this fluorochrome.

Staining and Buffer Recommendations

• When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:
  • Super Bright Complete Staining Buffer (Product # SB-4401-42) or
  • Brilliant Stain Buffer™ (Product # 00-4409-75)
• Light sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored up to 3 days at 4°C in:
  • IC Fixation Buffer (Product # 00-8222-49) or
  • 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

IL-3Ra (Interleukin-3 receptor subunit alpha) is part of the type I cytokine receptor family.
It forms a heterodimer with the common beta chain (CDw131) for IL-3 signal transduction.
Heterodimerization is induced by IL-3 and is essential for receptor activation.
IL-3Ra subunits are reported to be overexpressed in acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells, making them potential therapeutic targets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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