Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), a 145-kDa tyrosine kinase receptor expressed on most hematopoietic progenitor cells, mast cells, and other cell types. The interaction between c-Kit and its ligand (Steel factor) promotes proliferation and differentiation of hematopoietic progenitor cells.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.5 µg per test in 100 µL final volume. Optimize titration for best results.

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for instruments with a 355 nm UV laser. Protect from light due to photo-induced oxidation sensitivity.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Fixation:
Samples may be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness.

Excitation / Emission / Laser:

• Excitation: 350 nm
• Emission: 660 nm
• Laser: Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates and used under license. Powered by Sirigen™.

Target Information

KIT (c-KIT):
A proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (stem cell factor). KIT regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations are linked to gastrointestinal stromal tumors, mast cell disease, and leukemia. c-Kit activation triggers autophosphorylation and downstream signaling through Ras/ERK, PI3K, PLC-γ, and JAK/STAT pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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