Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Functional Grade, eBioscience

Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugates

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615
• Brilliant Violet 421, 480, 605, 711
• eFluor 450, 506
• FITC
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30–38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM).
KLRG1 is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with Ly49 molecules and SHP-1 signaling.

Applications Reported: Flow cytometric analysis and functional assays.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤1 µg/test (100 µL final volume).
Cell number should be determined empirically (10⁵–10⁸ cells/test). Titrate for optimal performance.

Storage and Handling: Use in sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family containing ITIM motifs in its cytoplasmic domain. These motifs interact with SH2 domains of phosphatases such as SHP-1.
KLRG1 functions as an inhibitory receptor expressed on NK cells and certain T cells, involved in tumor and virus-infected cell lysis and immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.