Takara TaKaRa Ex Premier DNA Polymerase

TaKaRa Ex Premier DNA Polymerase is a novel PCR polymerase derived from Thermococcus sp. The enzyme and buffers in this premix have been optimized to improve the PCR success rate for GC- or AT-rich sequences, long targets, and crude samples that are difficult to amplify with general PCR reagents. Furthermore, it has the high-fidelity of an α-type DNA polymerase and can be used for cloning.

This 2X premix contains all the components necessary for PCR (enzyme, buffer, dNTP mix, etc.) and does not freeze at –20°C, making the setup quick and easy. Simply add your primers and templates and run the PCR. The polymerase included is a hot-start polymerase that uses a monoclonal antibody to prevent non-specific amplification due to mispriming or the presence of primer-dimers.

Overview

• Faster amplification—amplifies targets at 30 sec/kb
• Higher success rate—easy amplification of difficult targets (long, GC/AT rich, or both)
• Ease-of-use—premix format with all necessary components (enzyme, buffer, dNTP mix, etc.), including a green gel-loading dye option for use in gel electrophoresis

More Information

PCR products

PCR products amplified with Takara Ex Premier DNA Polymerase have blunt end termini. To clone amplified products into T-vectors (TA cloning), dA addition to the 3` end is needed.

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.