Takara Capturem high-capacity membranes

Capturem nickel-functionalized membranes for his-tagged purification are a revolutionary solution for recombinant protein purification that effortlessly outperforms conventional methods. Novel nylon membranes have been assembled into spin columns and filtration devices, creating no-waiting, room-temperature workflows (just 5–30 minutes from loading a sample through elution) compatible with both mammalian and bacterial cells.

Capturem nickel-functionalized membranes for his-tagged purification are a revolutionary solution for recombinant protein purification that effortlessly outperforms conventional methods. Novel nylon membranes have been assembled into spin columns and filtration devices, creating no-waiting, room-temperature workflows (just 5–30 minutes from loading a sample through elution) compatible with both mammalian and bacterial cells. Exceptional performance is consistent under a wide range of research settings, including denaturing conditions, in the presence of additives (e.g., BME, EDTA, DTT, glycerol, TCEP, etc.; see compatibility table), and when performing tandem purification of his-tagged proteins.

The modified nylon membranes have a small bed volume, yet still produce high-purity, concentrated protein, due to the chemically enhanced surface area of the pores. With this technology, protein-binding capacity is comparable to, or better than, resins that bind 75 mg or more per cm3 of membrane. Miniprep, maxiprep, 24-well-plate, 96-well-plate, and large-volume formats are available to accommodate a range of input volumes. Furthermore, the short residence time of protein samples greatly reduces the possibility of protein degradation or loss of activity, giving downstream applications a higher probability of success.

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Overview

• No-waiting workflow—5-minute, room-temperature protocol
• High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
• Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
• Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
• Different cell systems—easy purification from mammalian and bacterial cells

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Applications

• Biochemical/enzymatic assays
• Pull-down or other protein interaction assays
• Confirmation screening
• Protein/ligand affinity analysis
• Crystallography or other structural analyses
• Antibody production
• Toxicology studies
• Protein transfer to live cells

Approximate yields for Capturem his-tagged formats

*Yields vary depending upon the sample type, species, and antibody isotype.

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.