Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H4), PerCP

Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H4), PerCP

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC
• FITC
• Request custom conjugation

Product Specific Information

1 µg/mL of MA5-45711 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs).
These HSEs are composed of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and located upstream of all heat shock genes.
Two HSFs have been identified in human cells, HSF1 and HSF2, which share 38% sequence identity and bind to the same HSEs.
HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
In unstressed cells, HSF1 exists as a monomer lacking DNA-binding activity. Upon stress, HSF1 is converted to a nuclear-localized trimeric form capable of DNA binding, and its phosphorylation is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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