Thermo Fisher Scientific SOX9 Recombinant Rabbit Monoclonal Antibody (7H13L8)
SOX9 단백질을 인식하는 토끼 유래 재조합 단일클론 항체로, Western blot, ICC/IF, ChIP 등 다양한 응용에 적합합니다. 높은 특이성과 일관된 로트 간 재현성을 제공하며, 인간 및 마우스 시료에 반응합니다. 단백질 A로 정제된 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2.5 µg/mL | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View 1 publication |
| ChIP assay (ChIP) | 2.5 µg/1x10^6 cells | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H13L8 |
| Immunogen | Peptides corresponding to human SOX9 (aa 4–17 and aa 47–62) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2716879 |
Product Specific Information
This antibody is predicted to react with Monkey and Pig, in addition to Human and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
SOX9 Function:
SOX9 plays a critical role in sex determination and differentiation of Sertoli cells. It is essential for chondrogenesis, regulating genes involved in cartilage formation as a transcription factor.
SOX9 translocation can cause campomelic dysplasia. It is involved in testis formation, neuron-glia differentiation in the spinal cord, and prostate epithelial development.
Additionally, SOX9 influences neural crest precursor formation and melanin production. It is expressed in adult testis, adult heart, and fetal brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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