Thermo Fisher Scientific NOD3 Polyclonal Antibody
Human NOD3 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. Affinity chromatography로 정제된 액상 항체이며, PBS/glycerol buffer에 보관. 면역계 관련 연구 및 NOD3 신호전달 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human NLRC3 (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.4), with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | −20°C, avoid freeze/thaw cycles, store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2854613 |
Product Specific Information
Positive Control:
Human thymus tissue lysates, HUVEC, MCF-7, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse colon tissue.
Target Information
NOD3 (also known as NLRC3) belongs to the NOD (nucleotide-binding oligomerization domain) family, which is involved in innate immune defense. NOD3 is predominantly expressed in the immune system, especially in T lymphocytes. Its expression is down-regulated following stimulation of the T-cell receptor complex and CD28, indicating a role in attenuating T-cell activation. NOD3 inhibits NF-κB, AP-1, and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or PMA/ionomycin treatment, and decreases IL-2 and CD25 mRNA induction in activated cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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