Thermo Fisher Scientific CRP Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific CRP Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CRP. Anti-Human CRP-specific antibody was purified by affinity chromatography and biotinylated.
• Sandwich ELISA: For detection of Human CRP, use 0.25–1.0 µg/mL of this antibody (100 µL/well). In conjunction with PeproTech Polyclonal Anti-Human CRP (500-P242) as a capture antibody, detects at least 2000–4000 pg/mL of Recombinant Human CRP.
• Western Blot: Use at 0.1–0.2 µg/mL for detecting Human CRP. Detection limit for Recombinant Human CRP is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
• Package: 500-P242BT-1MG is provided as 2 × 500 µg.
• AA Sequence: Discontinued.

Target Information

CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein composed of five identical, noncovalently bound, nonglycosylated subunits (206 amino acids each, MW 24 kDa). CRP is produced by the liver and its plasma levels rise sharply during inflammation. It initiates the classical complement cascade, binds chromatin and histones, and plays a key role in innate immunity and host defense. CRP acts as an opsonin, activates monocytes/macrophages, and may function as a scavenger during cell necrosis. Elevated CRP levels are associated with increased risk of hypertension and cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.