Thermo Fisher Scientific CRP Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CRP. Anti-Human CRP-specific antibody was purified by affinity chromatography using an immobilized Human CRP matrix.
• Sandwich ELISA: For detection of Human CRP using 100 µL/well, use 0.5–2.0 µg/mL of this antibody. When combined with PeproTech Biotinylated Anti-Human CRP (500-P242BT) as detection antibody, it allows detection of at least 2000–4000 pg/mL of Recombinant Human CRP.
• Western Blot: For detection of Human CRP, use 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
• AA Sequence: Discontinued.

Target Information

CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein composed of five identical, noncovalently bound, nonglycosylated subunits (each 206 amino acids, ~24 kDa). It is produced by the liver, and plasma levels rise dramatically during inflammatory processes.
CRP initiates the classical complement cascade, binds to nuclear components such as chromatin and histones, and plays a key role in innate immunity. It acts as an opsonin, activates monocytes/macrophages, and functions as a scavenger during cell necrosis.
Elevated basal CRP levels are associated with increased risk for hypertension and cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.