Thermo Fisher Scientific p14ARF Polyclonal Antibody
Human p14ARF 단백질을 인식하는 토끼 다클론 항체로, Western blot, IHC, ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관합니다. 종양 억제 단백질 연구 및 세포주 분석에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| ELISA | 1:100–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide to human p14ARF (C-terminal sequence) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Wet ice |
| RRID | AB_568664 |
Product Specific Information
Suggested positive control: BT549 or HeLa whole cell extracts, antigen standard for CDKN2A (transient overexpression lysate).
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins retinoblastoma protein and p53 tumor suppressors, respectively. Each has a role in the senescence of primary cells and activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression, which may contribute to the differing tumor susceptibility between species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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