Thermo Fisher Scientific CD43 Monoclonal Antibody (eBioR2/60), eBioscience
Mouse CD43 단백질을 인식하는 Rat IgM 형 단클론 항체로, Flow Cytometry, Western Blot, Immunoprecipitation에 사용 가능. 높은 순도(>90%)와 낮은 응집도(<10%)를 가진 액상 제품으로, 4°C 보관 및 Wet ice 배송. 다양한 세포 표면 CD43 아이소폼 검출에 적합.
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Thermo Fisher Scientific CD43 Monoclonal Antibody (eBioR2/60), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | ≤ 0.5 µg/test |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioR2/60 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_467240 |
Additional Formats
Product Specific Information
The eBioR2/60 monoclonal antibody reacts with mouse CD43 (leukosialin, sialophorin). CD43 glycoforms (~115 and 130 kDa) are expressed by early B cell subsets in bone marrow, thymocytes, mature T cells, NK cells, myeloid cells, and platelets. CD43 binds to CD54 and plays roles in adhesion and B cell survival.
Clone R2/60 detects both isoforms of CD43, unlike S7 and 1B11 clones that recognize distinct isoforms. R2/60 stains most CD4+, CD8+, and CD19+ splenocytes, indicating recognition of a shared epitope across CD43 isoforms.
Applications Reported: Flow cytometry, immunoprecipitation, and immunoblotting (WB).
(Use Functional Grade eBioR2/60, Product #16-0431, for functional assays.)
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal performance requires empirical titration.
| 품질 지표 | 기준 |
|---|---|
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Target Information
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein expressed on most hematopoietic cells. It contributes to a repulsive barrier affecting cellular adhesion and can promote leukocyte aggregation. Through interaction with actin-binding proteins (ezrin, moesin), CD43 regulates T-cell morphology and lymphocyte traffic.
CD43 signaling modulates LFA-1 adhesiveness and influences tumor-related pathways involving p53. Dysfunction of CD43 is associated with Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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