Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (SPM514)

Applications and Tested Dilution

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 upregulates transcription of genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, it includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerization domain, and a C-terminal regulatory domain.

Phosphorylation at Ser15 is crucial for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells and acts as an anticancer factor. It is also linked to aging, oxidative stress, and cognitive processes such as long-term potentiation (LTP).

In normal cells, p53 is expressed at low levels, but in transformed or malignant cells, expression increases significantly. Mutations in the TP53 gene are common in various human cancers and result in loss of tumor suppressor activity. Germline TP53 mutations are also associated with cancer-prone conditions such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.