Thermo Fisher Scientific ZO-1 Monoclonal Antibody (R26.4C), eFluor 570, eBioscience
ZO-1 단백질을 인식하는 R26.4C 클론의 형광 표지 단일클론 항체로, eFluor 570 결합. IHC, ICC/IF 등 다양한 형광 이미징에 적합. 556/569 nm의 발광 특성을 가지며, 세포 간 밀착연접 연구에 유용. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R26.4C |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573644 |
Additional Formats:
Product Specific Information
The monoclonal antibody R26.4C recognizes the tight junction protein ZO-1 (zona occludens) in rat, mouse, canine, and porcine.
ZO-1 is a member of the MAGUK family of tight junction–associated scaffolding proteins, containing PDZ, SH3, and GUK domains. It is an integral component of the tight junction complex, forming a barrier to paracellular movement of substances and maintaining epithelial cell polarity.
ZO-1 interacts with ZO-2, ZO-3, occludin, cingulin, claudins, actin, alpha-catenin, AF-6, ZONAB, Apg-2, and JAM-A–C, anchoring the actin cytoskeleton to the tight junction.
Down-regulation of ZO-1 and ZO-2 has been associated with cancer progression.
Applications Reported:
This antibody is suitable for immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry.
Applications Tested:
Validated for immunocytochemistry of formaldehyde-fixed and permeabilized cells at ≤1 µg/mL. Optimal dilution should be determined experimentally.
Filter Recommendation:
Use a filter capturing the 570 nm emission wavelength (e.g., Excitation 545/25, 565LP, Emission 605/70).
Standard Alexa Fluor® 555 or TRITC filters are acceptable.
Excitation: 555 nm
Emission: 570 nm
Target Information
ZO-1 forms complexes with ZO-2 or ZO-3 and associates with claudin, occludin, and F-actin at tight junction strands, linking the actin cytoskeleton to the junction.
ZO-1 expression is reduced in many breast cancer lines.
ZO-2 and ZO-3 are ubiquitously expressed in epithelial tight junctions, while ZO-1 is also expressed at cardiac myocyte junctions.
ZO-1 may be involved in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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