Thermo Fisher Scientific betacellulin Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Betacellulin. Anti-Human Betacellulin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hBetacellulin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Betacellulin (500-P254) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hBetacellulin.

Western Blot:
To detect hBetacellulin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant hBetacellulin is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P254BT-1MG is provided as 2 × 500 µg.

Target Information

The protein encoded by this gene belongs to the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is processed to a mature molecule by proteolytic events. This protein acts as a ligand for the EGF receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.