Thermo Fisher Scientific CTAGE5 Polyclonal Antibody, PeproTech
Human MIA-2 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western Blot 및 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관 가능합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 500-P255-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CTAGE5 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific CTAGE5 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MIA-2 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930092 |
Product Specific Information
AA Sequence of recombinant protein:
MLESTKLLAD LKKCGDLECE ALINRVSAMR DYRGPDCRYL NFTKGEEISV YVKLAGERED LWAGSKGKEF GYFPRDAVQI EEVFISEEIQ MSTKESDFLC L
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIA-2. Anti-Human MIA-2-specific antibody was purified by affinity chromatography employing an immobilized Human MIA-2 matrix.
Sandwich ELISA:
To detect Human MIA-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIA-2 (500-P255Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human MIA-2.
Western Blot:
To detect Human MIA-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIA-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CTAGE5/MIA-2 is a secreted cytokine and a member of the MIA/OTOR family. Members of this family, which also includes MIA, OTOR, and TANGO, share a Src homology-3 (SH3)-like domain. MIA-2 is predominantly expressed in hepatocytes. Elevated levels of MIA-2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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