Thermo Fisher Scientific CTAGE5 Polyclonal Antibody, PeproTech

Thermo Fisher Scientific CTAGE5 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MLESTKLLAD LKKCGDLECE ALINRVSAMR DYRGPDCRYL NFTKGEEISV YVKLAGERED LWAGSKGKEF GYFPRDAVQI EEVFISEEIQ MSTKESDFLC L

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIA-2. Anti-Human MIA-2-specific antibody was purified by affinity chromatography employing an immobilized Human MIA-2 matrix.

Sandwich ELISA:
To detect Human MIA-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIA-2 (500-P255Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human MIA-2.

Western Blot:
To detect Human MIA-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIA-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CTAGE5/MIA-2 is a secreted cytokine and a member of the MIA/OTOR family. Members of this family, which also includes MIA, OTOR, and TANGO, share a Src homology-3 (SH3)-like domain. MIA-2 is predominantly expressed in hepatocytes. Elevated levels of MIA-2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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