Thermo Fisher Scientific CTAGE5 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.5-2.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human MIA-2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CTAGE5,uniProtId:Q96PC5-3,ncbiNodeId:9606,antigenRange:20-119,antigenLength:1412,antigenImageFileName:500-P255-100UG_CTAGE5_Q96PC5-3_Rabbit.svg,antigenImageFileNamePDP:500-P255-100UG_CTAGE5_Q96PC5-3_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930092

Product Specific Information

AA Sequence of recombinant protein: MLESTKLLAD LKKCGDLECE ALINRVSAMR DYRGPDCRYL NFTKGEEISV YVKLAGERED LWAGSKGKEF GYFPRDAVQI EEVFISEEIQ MSTKESDFLC L.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIA-2. Anti-Human MIA-2-specific antibody was purified by affinity chromatography employing an immobilized Human MIA-2 matrix.

Sandwich ELISA: To detect Human MIA-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIA-2 (500-P255Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIA-2.

Western Blot: To detect Human MIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIA-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CTAGE5/MIA-2 is a secreted cytokine, and a member of the MIA/OTOR family. Members of this family, which also includes MIA, OTOR, and TANGO, share a Src homology-3 (SH3)-like domain. MIA-2 is predominantly expressed in hepatocytes. Elevated levels of MIA-2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.