Thermo Fisher Scientific NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal Antibody (SPM347)
Neuron Specific Enolase (NSE, gamma) 단클론 항체로 신경 및 신경내분비 세포 표지에 적합. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 인간 NSE 감마 서브유닛 특이적, 높은 민감도 및 특이성 제공. 연구용으로만 사용.
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Thermo Fisher Scientific NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal Antibody (SPM347)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.1–0.2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM347 |
| Immunogen | Synthetic peptide of human NSE gamma (aa 416–433; exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. NSE is stable in biological fluids and diffuses into extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.
NSE consists of alpha, beta, and gamma subunits:
- Alpha: expressed in most tissues
- Beta: expressed in muscle
- Gamma: expressed primarily in neurons and neuroendocrine cells
Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. NSE antibodies are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood serves as a biomarker for neuronal injury.
Clinically, NSE is used as a marker in neurological disorders such as:
- Stroke
- Hypoxic brain damage
- Status epilepticus
- Creutzfeldt-Jakob disease
- Herpetic encephalitis
Elevated NSE levels are also found in plasma and certain cancers, including pediatric neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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